A new marker on chromosome 15, 1115S74, detects three RFLPs

Evolving Molecular Marker Technologies in Plants: From RFLPs to GBS

Analysis of DNA-sequence variation (or allelic state) at a specific chromosomal location in an individual/genotype is referred to as genotyping. Variation in the DNA sequence may or may not have functional significance. For example, variation may result either in a synonymous or non-synonymous change in a codon. Such alterations may either cause a favorable change or deleterious mutations (mis-...

Human cDNA probe (OL167,D21S109) detects RFLP on chromosome 21.

Two 47z [DXYS5] RFLPs on the X and the Y chromosome.

Source and Description 47z (DXYS5), located on the short arm of Ychromosome, i s a 2.3Kb EcoRI/Mbol fragment inserted in to the EcoRI s i t e of pBR322 with a BamHI/EcoRI l inker (1 ) . Homologous sequence i s located on Xq21. Polymorphism 1. Y-chromosome polymorphism: StuI iden t i f i e s a two-al lele polymorphism at 17Kb and 5.3Kb. 2. X-chromosome polymorphism: Hindlll-TaqI double digestion...

Sperm analyses, genetic counselling and therapy in an infertile carrier of a supernumerary marker chromosome 15.

PURPOSE A supernumerary marker chromosome (SMC) was analysed after lymphocyte culture of a patient with oligoasthenoteratozoospermia (OAT) before ICSI treatment. MATERIAL AND METHODS By additional molecular cytogenetic investigations the marker could be identified as a heterochromatic derivate of chromosome 15 [karyotype: 47,XY,+der(15)]. RESULTS Sperm analyses by interphase FISH showed a n...

Two RFLPs in human inter-alpha-trypsin inhibitor heavy chain gene ITIH2 on chromosome 10.

SOURCE AND DESCRIPTION OF CLONE : The 0.8 kb Eco RI / Bam HI fragment of lambda HuHITI-9 (1) used as a probe codes for human heavy chain H2 of the inter-alpha-trypsin inhibitor (2). POLYMORPHISMS : Kpn 1 (GGTAC/C) identifies one invariant band at 8.5 kb and a diaJlelic polymorphism with DNA fragments at 26.0 kb or 20.0 kb. Msp I (C/CGG) identifies three invariant bands at 2.35 kb, 2.1 kb and 1....